高温对淮南麻黄鸡血脑屏障的影响

doi:10.11843/j.issn.0366-6964.2017.04.021

摘要/Abstract

摘要：

为探究高温对淮南麻黄鸡血脑屏障的影响，将120羽107日龄淮南麻黄鸡随机均分为四组：常温对照组（Ⅰ组）、常温注射组（Ⅱ组）、高温对照组（Ⅲ组）、高温注射组（Ⅳ组）。Ⅰ、Ⅱ组于常温下饲养（21~23℃、RH 60%~70%），Ⅲ、Ⅳ组每天高温处理3 h（34~37℃、RH 45%~50%），试验持续2周。试验第7、14天，每组随机抽取5羽鸡，Ⅱ、Ⅳ组鸡一次静脉注射γ-氨基丁酸（GABA）（每千克体重8.87 mg），Ⅰ、Ⅲ组鸡注射同等剂量的生理盐水，注射0.5 h后断头取脑，冰浴上迅速采集脑组织。使用ELISA法分别检测第7、14天四组鸡脑组织中的GABA含量；采用电镜技术观察第14天常温对照组和高温对照组中鸡脑组织的血脑屏障超微结构，并用qRT-PCR技术和Western blot技术检测其脑组织中紧密连接蛋白ZO-1和Occludin的mRNA及蛋白质的表达量。结果显示：试验第7天，Ⅰ、Ⅱ组鸡脑组织中GABA含量无显著差异（P>0.05），Ⅳ组鸡脑组织中GABA含量极显著高于Ⅲ组（P<0.01）；试验第14天，Ⅰ、Ⅱ组间鸡脑组织中GABA含量无显著差异（P>0.05），Ⅳ组鸡脑组织中GABA含量高于Ⅲ组，但差异不显著（P>0.05）。与常温对照组相比，高温对照组鸡的血脑屏障结构受到破坏，电镜下观察到大脑皮层血管内皮细胞线粒体嵴有的断裂、有的空泡变性，血管内皮细胞间紧密连接间隙增宽，内皮细胞外基膜增厚、结构疏松；高温对照组鸡脑组织ZO-1的mRNA相对转录量极显著低于常温对照组（P<0.01），Occludin 的mRNA相对转录量显著低于常温对照组（P<0.05）；高温对照组鸡脑组织ZO-1和Occludin蛋白的相对表达量极显著低于常温对照组（P<0.01）。高温破坏了鸡的血脑屏障，脑组织中ZO-1和Occludin表达下降可能是其原因之一。

Abstract:

The aim of the present study was to explore the impact of high temperature on Huainan partridge chickens’ blood-brain barrier. One hundred and twenty 107-day old Huainan partridge chickens were randomly divided into four groups: chickens in group Ⅰ and Ⅱ were raised at normal room temperature (21-23℃, RH 60%-70%), which in Group Ⅲ and Ⅳ subjected to hyperthermia (34-37℃, RH 45%-50%) for 3 hours daily. The treatment lasted for two weeks. Five chickens were randomly selected from each group on day 7 and 14, respectively, chickens in group Ⅱ and Ⅳ were received a single intravenous dose of Gamma-aminobutyric acid (GABA) (8.87 mg·kg^-1 body weight), chickens in group Ⅰ and Ⅲ received same dose of saline, brain tissue samples were collected at time 0.5 h and analyzed GABA using ELISA assay. The brain tissue samples were collected on day 14, and the ultrastructure of the blood brain barrier were detected using transmission electron microscopy (TEM), the mRNA and protein expression of ZO-1 and Occludin in the brain tissues were detected by qRT-PCR and Western blot. The results showed that, On day 7 and 14, no significant differences of brain GABA concentration were found between group Ⅰ and Ⅱ (P>0.05). Brain GABA concentration of chicks in Group Ⅳ were higher than that in group Ⅲ on day 7 and 14, and the differences were very significant on day 7 (P<0.01). Compared with normal temperature, high temperature destroyed the blood-brain barrier structure of chickens. Mitochondrial cristae of Cerebral cortical capillary endothelial cells were damaged, some broken, some degeneration. Capillary endothelial cell tight junctions gap widened. Microvascular basement membrane became loose and thick. The mRNA transcription of ZO-1 and Occludin were very significantly (P<0.01) and significantly lower (P<0.05) than those in normal temperature group, respectively. The protein relative expression of ZO-1 and Occludin were very significantly (P<0.01) lower on days 14 than those in normal temperature group. High temperature destroyed the chicken blood-brain barrier, ZO-1 and Occludin down-regulation may be one of the mediators of it.

中图分类号:

S852.3

姜丹, 冯士彬, 王希春, 李玉, 詹凯, 吴金节, 李锦春. 高温对淮南麻黄鸡血脑屏障的影响[J]. 畜牧兽医学报, 2017, 48(4): 769-776.

JIANG Dan, FENG Shi-bin, WANG Xi-chun, LI Yu, ZHAN Kai, WU Jin-jie, LI Jin-chun. Effects of High Temperature on Huainan Partridge Chickens' Blood-brain Barrier[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(4): 769-776.

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